Abstract
Antibodies against the globular domain of histones H1° and H5 were developed in rabbit. The antibody against the globular domain of H5 cross-reacted with H1° but not with H1; the antibody against the globular domain of H1° did not cross-react with H5, H1 or with HMG proteins. The globular domain of H1° therefore appears to have an immunological determinant(s) which does not exist in H1 and H5. By use of these antibodies, we show that nucleated erthrocytes of bullfrog contain an H1°-like protein (not an H5-like protein). This observation coincided with the report of Shimada, T. [J. Biol. Chem. 256 (1981) 10577–10582]. These antibodies have application in detecting H1°-like proteins in eukaryotic cells.
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