Antibodies to the calmodulin-binding Ca 2+-transport ATPase from smooth muscle

Abstract

Antibodies were raised against a calmodulin-binding CaMg-ATPase (Ca 2+-transport ATPase) from smooth muscle. The binding of these antibodies to a number of related Ca 2+-transport ATPases was studied. Antibodies to the calmodulin-binding ATPase from porcine antrum (stomach) smooth muscle do not only bind to this CaMg-ATPase, but also to the corresponding enzyme in porcine erythrocytes. However, they do not bind to the CaMg-ATPase from sarcoplasmic reticulum of porcine skeletal muscle. The binding of these antibodies to the CaMg-ATPase of smooth muscle, does not inhibit the enzyme activity.