Abstract
Parenteral immunization with semen, with Nagase egg-yolk diluent (NEYD) or with semen in NEYD depressed fertility in does artificially inseminated with semen in NEYD. Although fertilization rates were significantly reduced in the animals which had been immunized with semen alone (P < 0.05) or with diluent alone (P < 0.05), the greatest reduction occurred in the animals which had been immunized with semen plus diluent (P < 0.01). Both the anti-semen antibodies and the anti-diluent antibodies contributed to the reduction in fertility of the animals which had been immunized with semen plus diluent.
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