Abstract
Antisera from rabbits that had been immunized with a highly active membrane preparation of (Na + + K +)-ATPase from the outer medulla of pig kidney strongly inhibited (Na + + K +)-ATPase activity in various tissues. When the antiserum was incorporated into released human red cell ghosts, the ouabain-sensitive efflux of Na + into both 15 mM K + and K +-free high Na + media was completely abolished. This effect was not observed when non-immune serum was used, or when the immune serum was allowed access only to the outer surface of the red cell membranes.
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