Antibodies to Heat Shock Proteins 90\u03b1 and 90\u03b2 in Psoriasis

Aleksandra Damasiewicz-Bodzek,Magdalena Szumska,Krystyna Tyrpień-Golder

doi:10.1007/s00005-020-00573-7

Aleksandra Damasiewicz-Bodzek, Magdalena Szumska + Show 1 more

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https://doi.org/10.1007/s00005-020-00573-7

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Abstract

One of many hypotheses of psoriasis pathogenesis supposes an overexpression of heat shock proteins (Hsps) in different skin layers and systemic immunologic response to them. Hsp90 is one of the most abundant chaperone in eukaryotic cells. The number of studies concerning the role of Hsp90 and anti-Hsp90 antibodies in etiopathogenesis of various diseases is also constantly expanding. Still, there are not many reports concerning potential involvement of this Hsp family or anti-Hsp90 immunization in pathomechanism of psoriasis. The aim of the study was the estimation of anti-Hsp90α and anti-Hsp90β IgG antibodies in the sera of the psoriatic patients at different phases of disease activity in comparison to the sera of healthy individuals. The study material consisted of sera from psoriasis patients (n = 80) in active phase and in the remission phase and healthy individuals (n = 80). Concentrations of anti-Hsp90α and anti-Hsp90β IgG antibodies were determined using ELISA technique. In the patients with psoriasis (both in the active phase of the disease and in the remission phase) concentrations of anti-Hsp90α antibodies were significantly higher than in healthy individuals and they correlated positively with psoriasis area severity index values. The mean concentrations of anti-Hsp90β antibodies in the psoriatic patients and healthy controls were comparable. The obtained results indicate an existence of increased immunological response to Hsp90α in psoriasis. It may suggest the role of the extracellular form of this chaperone and/or anti-Hsp90α antibodies in etiopathogenesis of this dermatosis. The inhibition of Hsp90α may represent a novel therapeutic approach to treat psoriasis.

Highlights

The etiopathogenesis of psoriasis is very complex and has not been fully known, which results in serious therapeutic problems
Mean concentrations of antiHsp90β antibodies in the psoriatic patients did not differ significantly from those observed for healthy individuals and these concentrations did not differ between the active phase and remission phase of disease
The concentrations of anti-Hsp90α antibodies in the active phase showed a moderate positive linear relationship with the concentrations of anti-Hsp90α antibodies in the remission phase of psoriasis (R = 0.58; p = 0.000000) (Fig. 3c) and the concentrations of anti-Hsp90β antibodies in the active phase showed a strong positive linear relationship with the concentrations of anti-Hsp90β antibodies in the remission phase of psoriasis (R = 0.74; p = 0.000000) (Fig. 3d)

Summary

Introduction

The etiopathogenesis of psoriasis is very complex and has not been fully known, which results in serious therapeutic problems. One of many hypotheses of psoriasis pathogenesis supposes an overexpression of heat shock proteins (Hsps) in different skin layers and systemic immunologic response to these proteins (Ghoreishi 2000). Heat shock proteins are cellular chaperones (prevents irreversible denaturation and aggregation of cellular proteins destroyed by stress conditions). They are responsible for the balance of intracellular redox potential, stabilization of the cytoskeleton and regulation of complicated processes of cellular proliferation and differentiation, apoptosis, and oncogenesis. Heat shock proteins are highly conserved protein found in very different species such as bacteria and mammals.

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