Abstract

Immunization of rabbits with a preparation of DNP-insulin, specially purified by isoelectric focusing, has led to the production of anti-DNP antibody of very restricted heterogeneity in all seven rabbits examined and in amounts up to 800 μg/ml serum. The homogeneity of one of the antibody samples was shown by the straight line character of its binding curve for 3H-labeled DNP-lysine (Sips' heterogeneity index = 1.0) and by the simplicity of its isoelectric focusing pattern. Similar simple patterns were observed for antibodies from the other six rabbits although two produced very little anti-DNP antibody.

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