Antibodies from phage display libraries as immunochemical reagents.

Abstract

AbstractThe display of a repertoire of recombinant antibodies on the surface of filamentous phage by fusion to a minor coat protein has provided a powerful methodology for isolating the desired binding specificities (see Chapter 45 and ref. 1). Antibodies have typically been displayed on phage as single-chain Fv fragments (scFv [2]), in which the heavy chain variable region (VH) and the light chain variable region (VL) domains are linked together by a flexible polypeptide (3,4), or as Fab fragments (5) In general, antibodies have been displayed either at the N-terminus of the minor coat protein pill of filamentous phage (2), or at the C-terminal domain of pill (6). For Fab fragments, one chain is fused to pill, and the other assembles with the pill-fused chain after leader peptide-mediated secretion in the bacterial supernatant.KeywordsAntibody FragmentPhage ParticleFilamentous PhageHelper PhagePhage ELISAThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.