Abstract
The anti-mouse IL-2-R beta chain mAb TM-beta 1 which, by itself, does not affect IL-2-dependent proliferation through the high affinity mouse IL-2 receptor, was shown to cooperate in a synergistic way with a set of anti-IL-2-R alpha chain mAbs both in vitro and in vivo. In vitro, when associated at equimolar concentrations, the TM-beta 1/anti-alpha mAb association was four to ten times more efficient at inhibiting the proliferation of the CTL-L2 cell line than was a similar concentration of anti-alpha mAb alone. In addition, a bispecific antibody in which a Fab' fragment of TM-beta 1 was covalently linked to a Fab' fragment of one of the anti-alpha mAb (5A2) was shown to be as efficient as the TM-beta 1/5A2 association. The association of TM-beta 1 with 5A2 was also tested in vivo in a sheep red blood cell-induced delayed type hypersensitivity (DTH) model. TM-beta 1 which, by itself, had no effect on DTH, induced a two- to threefold decrease in the doses of 5A2 required to suppress this cell-mediated immune reaction.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Transplant international : official journal of the European Society for Organ Transplantation
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.