Abstract
Approximately 50 toxic antibiotics were tested as possible inhibitors of respiratory and phosphorylating systems in mitochondria and of glycolysis by Ascites tumor cells. Oligomycin, Nigericin, and Dianemycin inhibited respiration and phosphate uptake in the presence of several substrates. Each of these agents displayed considerable selectivity in these systems as well as in their inhibition of mitochondrial adenosinetriphosphatase (ATPase). Oligomycin inhibits, virtually completely, the ATPase and the ATP-P i 32 exchange reaction in mitochondria and in submitochondrial particles. The effects of Nigericin and Dianemycin are dependent to some extent on the structural integrity of the mitochondria. Two antibiotics which are known to affect electron-carrying systems were found to inhibit, partially, anaerobic glycolysis in Ascites tumor cells. The results affirm the general utility of toxic antibiotics as tools for metabolic investigations.
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