Antibiotic susceptibility pattern of Salmonella isolates from enteric fever suspected patients

Abstract

Background Enteric fever is one of the most common diseases encountered worldwide and is endemic in Nepal. This study was conducted to access antibiotic susceptibility pattern of Salmonellaisolates from culture positive cases of enteric fever. Methods Altogether 505 blood samples were collected from patients clinically suspected of enteric fever attending HAMS Hospital. All blood samples were cultured by BACTEC method and sub cultured in blood agar and MacConkey agar plates. All isolates were identified by colony characteristics, biochemical tests and serotyping methods. Antibiotic susceptibility test was performed by modified Kirby Bauer disc diffusion method interpreted with CLSI guideline. Result Isolation rate of Salmonellaspecies was 3.6 %. Among 18 Salmonellaisolates, 10 were S. typhi, 8 were S. paratyphi A. The prevalence rate of infection was high among the age group 11–20 years (50 %) and among the male patients. However, there was no significant association of enteric fever with gender of patients (P=2.47). All 18 isolates were sensitive to Amoxycillin, Azithromycin, Ceftriaxone and Chloramphenicol, Ciprofloxacin and Ofloxacin. Majority of isolates were sensitive to Cefixime (94.4 %), Cotrimoxazole (94.4 %) and Cephotaxime (90 %). There were no any MDR isolates. Higher percentage of isolates was resistant to Nalidixic acid (87.5 %). Conclusion The decreased susceptibility to Fluroquinolones of S. typhi and S. Paratyphi A can be correlated with resistance to Nalidixic acid. Commonly used third generation Cephalosporins and rolled back first line drugs be the choice in case of NARS isolates.