Antibiotic Susceptibility and Molecular Detection of Pseudomonas aeruginosa Isolated from Bovine Mastitis

Hala S.R Al-Taee,Hazim I Al-Ahmed,Ikram A.A Al-Samarraae

doi:10.30539/iraqijvm.v43i2.536

Hala S.R Al-Taee, Hazim I Al-Ahmed + Show 1 more

Open Access

https://doi.org/10.30539/iraqijvm.v43i2.536

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Abstract

This study aimed to isolate Pseudomonas aeruginosa from cattle (bovine) milk with mastitis to characterize its antimicrobial susceptibility against some antibiotics, and to identify aminoglycoside acetyltransferase (aac-3-Ib) gene.A total of 100 bovine milk samples were collected randomly from different local cow farms at districts of Wasit governorate, Iraq. Six P. aeruginosa isolates were obtained using bacterial culture method and further identified by Analytical Profile Index (API-20E). The antibiotic sensitivity test was performed by disc diffusion methods. Among the 5 antibiotics used, the highest resistance (100%) was found with Nalidixic acid andtetracycline, follow by gentamicin (50%) and the lowest resistance rate (16.6%, and 33.3%) was to the ciprofloxacin and cephalothin respectively. PCR was performed for all the gentamicin resistant isolates that be among the aminoglycoside family. The where the frequency of aac(3)-Ib gene have product (530bp) was to 3 of P. aeruginosa isolates. From the finding of present study we concluded that P.aeruginosa thatisolated from mastitic bovinehave been developed resistance against aminoglycosides through presence of aac(3)-Ibgene and the ciprofloxacin and cephalothin can be taken as good choice of treatment

Highlights

Pseudomonas aeruginosa (P. aeruginosa) is one of the top 10 superbugs in the world, causing infections with bad condition in human and animal [1]
The six enzymes produce by six genes (AAC-6'-I), (AAC-6'-II), (AAC-3'-Ia), (AAC-3'-Ib), (AAC-6'-IIb) and (APH-3'-VI) [11] are the most commonly changed enzymes in P. aeruginosa, and their substrates are the most common and most important against pseudo aminoglycosides
This study aimed to isolate P. aeruginosa from cattle infected with mastitis with detection of aminoglycoside acetyltransferase gene that is responsible for gentamicin resistance among aminoglycoside group

Summary

Introduction

Pseudomonas aeruginosa (P. aeruginosa) is one of the top 10 superbugs in the world, causing infections with bad condition in human and animal [1]. Due to the spread of antimicrobial-resistant strains, therapeutic options are still severely limited; infection with P. aeruginosa remains a life-threatening risk [2]. Serious infections, both acute and chronic, are always nosocomial and correlated with compromised host defenses; but, this opportunistic pathogen is increasingly identified as the cause of disease in both livestock and fellow animals, these include otitis and urinary tract infections in dogs and cats, mastitis in dairy aminoglycoside phosphoryl transferase (APH) that act on plasmid or chromosome genes. The six enzymes produce by six genes (AAC-6'-I), (AAC-6'-II), (AAC-3'-Ia), (AAC-3'-Ib), (AAC-6'-IIb) and (APH-3'-VI) [11] are the most commonly changed enzymes in P. aeruginosa, and their substrates are the most common and most important against pseudo aminoglycosides. This study aimed to isolate P. aeruginosa from cattle infected with mastitis with detection of aminoglycoside acetyltransferase (aac-3-Ib) gene that is responsible for gentamicin resistance among aminoglycoside group

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