Antibiotic Sensitivity on Isolates from Diabetic And Non-Diabetic Population Based In Pattoki Punjab

Abstract

Objectives: To isolate and characterizing microorganisms from diabetic and non-diabetic patients and assessing the antimicrobial activity of isolate extracts from Citrus sinensis and Psidium guajava. Methodology: Experimental study of one year and six month duration from May 2016 to May 2018 conducted in Microbiology Laboratory University of Lahore, Pakistan. Convenient sampling was done by collecting the urine sample at random from 250 persons in the Pattoki community. All the urine samples from diabetic and non-diabetics were cultured separately and after biochemical confirmation of microorganisms, the disc diffusion method was used for carrying out an antimicrobial activity.Results: A total of 105 samples, were found to have positive urine cultures. Among these positive urine culture samples, 75 were diabetic and 30 were non-diabetics. The bacterial isolate most commonly found among diabetics was E.coli followed by the presence of Staphylococcus aureus, Klebsiella pneumoniae,Pseudomonas aeruginosa, Proteus vulgaris respectively. Minimal Inhibitory Concentration (MIC) of different parts of plant extracts against isolated bacteria from diabetics showed that the highest sensitivity was shown against peel, leaves and seed extracts of Citrus sinensis and Psidium guajava while the least sensitivity was shown against the stem and root extracts of these plants. Moreover, antibiotic sensitivity tests of isolated microbes showed that the highest resistance is found against Augmentin and Tetracycline while the most sensitive drug for isolates was found to be Chloramphenicol.Conclusion: From the results, it is concluded that leave extract of Psidium guajava and peel extracts of Citrus sinensis showed effective results against bacterial pathogens and could serve as a good alternate source of antibacterial agents