Abstract

 
 
 
 
 Abstract 
 
 Proteus is an important microorganism that have been isolated from wound infections causing severe complications. This study was aimed to isolate and determine the antibiotic sensitivity profile against isolates. A total of 50 wound and pus samples were collected from patients admitted in BMC Hospital, Quetta and processed in Pathobiology Laboratory of Center for Advance Studies of Vaccinology and Biotechnology (CASVAB) University of Baluchistan. All the samples were inoculated onto Brain Heart infusion (BHI), MacConkey agar, Cysteine-Lactose-Electrolyte-Deficien (CLED) and Blood agar and incubated at 37 oC, overnight. The suspected colonies were identified by gram stain and different biochemical tests and also confirmed by local made Quick Test Strip 12 (QTS 12). Among the total 50 samples 8 were found positive for Proteus mirabilis. Antimicrobial susceptibility of Proteus mirabilis against different antibiotic was evaluated by Kirby-Baur Method. Four different antibiotics were tested and ampicillin was found highly susceptible (75%), followed by Gentamicin (50%), Ceftazidime (40%) and ciprofloxacin (40%). This study shows the incidence of Proteus mirabilis in wound infection and best possible antimicrobial therapies against its infection.
 
 
 
 
 
 
 
 
 
Highlights
Genus Proteus is a member of family Enterobactericeae
Out of 50 clinical samples total of 8 isolates were confirmed as Proteus mirabilis by different biochemical tests as shown in (Table I)
All the 8 purified triple cloned isolates of Proteus mirabilis were subjected to different test like Indole, Methyl red, Vogas prausker, Citrate utilization, H2S gas production, Triple sugar iron, nitrate reduction, Oxidase, Catalase, urease reactions and results of all biochemical test are illustrated in (Table ll)
Summary
Genus Proteus is a member of family Enterobactericeae. Many species are member of genus Proteus i.e. Proteus mirabilis, Proteus hauseri, , Proteus penneri and Proteus vulgaris. This genus is usually Saprophytic in nature but the Many Member of this genus are opportunistic pathogens ie Proteus mirabilis, Proteus penneri and Proteus vulgaris. These are involved in septic diseases, nosocomial and genito urinary tract infections [1]. Proteus is part of normal flora of Gastrointestinal tract It is wide spread in nature and is 3rd major causitive agent of nosocomial (hospital acquired infections) [2,3]
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