Abstract
BackgroundThe way of treating different types of infectious diseases is really important. Using genotyping method, we can determine the genetic relatedness between the organisms with different resistance profile from different sources. The aim of this study was to determine antibiotic resistance and genotyping of uropathogenic Escherichia coli (UPEC) strains using pulsed field gel electrophoresis (PFGE).MethodEscherichia coli (E. coli) strains were recovered from the patients with urinary tract infections (UTI) whom admitted in several major hospitals in Tehran. Antibiotic susceptibility testing was done according to CLSI guideline. The present of some virulence factor have been detected using PCR assay. Genotyping of the strains was performed by PFGE and all PFGE profiles were subjected to data processing.ResultIn total, 60 E. coli strains were subjected to the study. Most of E. coli isolates were resistant to cefepime (100%) and cephalothin (74%) and susceptible to imipenem (100%), vancomycin (100%) and doxycycline (100%). Among the UPEC isolates the prevalence of fimbriae type I (fimH), hemolysin (hlyA) and aerobactin (aer) genes were 89%, 60% and 90%, respectively. The PFGE differentiated E. coli strains into 33 different genetic clusters. Majority (30%) of them including PFGE type 11 generated 15 bands, while PFGE type 2 was the lowest (2%) prevalent group with 9 bands.ConclusionThe result showed that the antibiotic resistance is escalating rapidly. UPEC strains causing infections are more likely to harbor certain virulence genes. Our finding also showed E. coli strains isolated under the study were belonged to the diverse clones.
Highlights
The way of treating different types of infectious diseases is really important
The result showed that the antibiotic resistance is escalating rapidly
Bacterial isolates and antibiotic susceptibility testing Over 6-month period, a total of 60 E. coli strains were isolated and subjected to this study
Summary
The way of treating different types of infectious diseases is really important. Using genotyping method, we can determine the genetic relatedness between the organisms with different resistance profile from different sources. The aim of this study was to determine antibiotic resistance and genotyping of uropathogenic Escherichia coli (UPEC) strains using pulsed field gel electrophoresis (PFGE). Some of the virulence genes of UPEC strains are aerobactin (aer), P fimbriae (pap), hemolysin (hly), type 1 fimbriae, afimbrial adhesin I (afa I), In the past recent years, the spectrum and frequency of antimicrobial-resistant UTIs have raised [6, 7]. The resistant patterns of bacteria have been varied by geographical location and by time so periodically testing of antibiotic resistant is really important. E. coli strains are the leading causes of serious bacterial infections in health society and very different antibiotic patterns have been reported based on the source [8, 9]. E. coli can be intrinsically resistant to some special antibiotics and have gens which are responsible for resistance to some of antibiotics such as aminoglycosides, flouroquinolones and β-lactamas [10, 11]
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