Antibiotic resistance profiling of Uropathogenic Enterobacteriaceae, Casablanca, Morocco

Aniba Rafik,Timinouni Mohammed,Nayme Kaotar,Barguigua Abouddihaj,Dihmane Asmaa,S Bourekkadi,A Soulaymani,A Mokhtari,H Hami,K Slimani

doi:10.1051/e3sconf/202131901002

Aniba Rafik, Timinouni Mohammed + Show 8 more

Open Access

https://doi.org/10.1051/e3sconf/202131901002

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Abstract

Introduction:the urinary tract infection is a pathology frequents as well in community as at the hospital. In last years, there has been increased the incidence of antibiotic resistance in Uropathogenic Enterobacteriaceae. This development explains the need for periodic regional surveillance of this resistance to antibiotics, which poses a real problem of public health, because it affects several molecules ranging from β-lactam to quinolones.Objective:the aims of this study is to determine the profile of antibiotic resistance of Uropathogenic Enterobacteriaceae isolated from medical analysis laboratories in Casablanca, Morocco.Materials and methods:This is a prospective study that was conducted on patients who visited medical analysis laboratories in Casablanca city, for urinalysis test from 01 January 2017 to 30 December 2018 (a two full years). The culture was performed according to the usual techniques, and the antibiogram was done according to the recommendations of the Antibiogram Committee of the French Society of Microbiology. The statistical analysis was performed using Microsoft Excel (Microsoft 2016).Results: During our study, we reported 18% of urinary tract infections, Sex ratio F/M was 1.79. In addition, Enterobacteriaceae were the most common germs. Of all the strains isolated, we noted a predominance ofEscherichia coliwith 75.41%) andKlebsiella sspwith 17.05%.In the course of this study period, we observed high rates of resistance to the main classes of antibiotics, and an overall increase between 2017 and 2018 in this resistance to the majority of β-lactam antibiotics, quinolones and aminoglycosides for almost all species of Uropathogenic Enterobacteriaceae. However, amikacin was the most active molecule against the Uropathogenic Enterobacteriaceae strains isolates.Conclusion:The development of resistance of Uropathogenic Enterobacteriaceae to antibiotics is a worrying phenomenon that exposes us to difficulties in the therapeutic management of infections. The current control of antimicrobial resistance is a real emergency and requires the involvement of public awareness before government instructions.

Highlights

Urinary tract infections (UTIs) are a severe public health problem and are caused by a range of pathogens, but most commonly by Enterobacteriaceae family: Escherichia coli, Klebsiella spp, Proteus spp, Enterobacter spp [1]
The development of resistance of Uropathogenic Enterobacteriaceae to antibiotics is a worrying phenomenon that exposes us to difficulties in the therapeutic management of infections
The ascending pathophysiology of urinary tract infection and the strong colonization of the perineum by Enterobacteriaceae of digestive origin, and in particular Escherichia coli, associated with specific factors of uropathogenicity such as bacterial adhesins capable of binding to the urinary epithelium explain that the species Escherichia coli dominate the epidemiological profile with 75, 41 % flowed by Klebsiella spp strains with 17.05%

Summary

Introduction

Urinary tract infections (UTIs) are a severe public health problem and are caused by a range of pathogens, but most commonly by Enterobacteriaceae family: Escherichia coli, Klebsiella spp, Proteus spp, Enterobacter spp [1]. The widespread and misuse of these antibiotics resulted in the increasing rate of resistance to them in the societies [3]. The increase in rates of antibiotic resistance among Enterobacteriaceae has posed challenges in choosing regimens, especially when infection due to multidrug resistant Enterobacteriaceae are suspected or endemic. It’s become a major international public health issue, raising fears of therapeutic impasses, that’s why it has the priority for surveillance and resistance studies given their high frequency and severity [4, 5]. In Morocco, the data on resistance to antibiotics of Uropathogenic Enterobacteriaceae are very limited and needs further investigations [6, 7]

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