ANTIBIOTIC RESISTANCE PROFILE AND MOLECULAR CONFORMATION AND MRSA ISOLATE BY MULTIPLEX PCR FROM CLINICAL SAMPLE AT A TERTIARY CARE HOSPITAL IN INDIA

Abstract

Methicillin Resistant Staphylococcus aureus (MRSA) is an important human pathogen. They are emerging as a serious public-health issue. It causes nosocomial and community acquired infections. MRSA is present throughout hospitals in many countries. Nowadays it is the most commonly isolated antimicrobial resistant pathogen worldwide. The study include Material Method – d 450 isolates of Staphylococcus species from various clinical samples. The samples received from outpatient departments (OPDs), inpatient departments (IPDs) & various intensive care units (ICUs) included urine, blood, pus, sputum, vaginal swab, tissue, pleural uid. All clinical samples were processed as per standard bacteriological techniques. Molecular identication of Antibiotic Resistance Gene-mecA gene was also performed. Out of 450 Result– Staphylococcus species, 150 isolates were Staphylococcus aureus & 300 isolates were Coagulase Negative Staphylococci (CONS). Conclusion – Methicillin resistance is caused by the presence of mec- A gene, which encodes a low afnity penicillin binding protein (PBP)-2a or PBP2' which has a low afnity for β-lactam antibiotics. Therefore, presence of mec-A gene indicates methicillin resistance in Staphylococci. Detecting mec-A gene by polymerase chain reaction is now considered the gold standard for identifying methicillin resistance in S. aureus.