ANTIBIOTIC RESISTANCE PATTERN OF BACTERIAL PATHOGENS ISOLATED FROM PUS IN A TERTIARY CARE HOSPITAL OF CENTRAL INDIA

Abstract

Pyogenic infections are commonly caused by various microorganisms and mixed infections that require antibiotic therapy. Emergence of antimicrobial drug resistance among susceptible individuals due to injudicious use of antibiotics has made the treatment of wound infections very difcult. This study was undertaken to determine the antibiotic resistance pattern among aerobic bacteria isolated from pus samples. A total of 312 pus samples were received in the Department of Microbiology, GMCH Nagpur from January to March 2021. The pus isolates were identied and processed by standard bacteriological techniques. The Antimicrobial Susceptibility Testing (AST) was performed by Kirby-Bauer disc diffusion technique and the drugs used were in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines 2020.Out of total 312 pus samples, 182 (58.33%) yielded signicant bacterial growth. The most prevalent pathogen isolated were Klebsiella pneumoniae 60 (19.23%) followed by Escherichia coli 42 (13.46%). Further, the study shows that Methicillin Resistant Staphylococcus Aureus (MRSA) and Inducible Clindamycin Resistance (ICR) were 29.41% and 11.76% respectively; moreover 70% isolates were Multi Drug Resistant (MDR). Therefore, this study indicates the prevalence of resistance to different classes of antibiotics in bacterial isolates and highlights the need for effective surveillance, regular reporting and antibiogram-guided antibiotic prescription.