Abstract
Cholera is an acute form of the diarrheal disease that plagued human civilization over the centuries. The enormity of human sufferings led clinicians and scientists to carry out extensive research on cholera and Vibrio cholerae leading to major discoveries that opened up novel areas of research or new disciplines in biomedical sciences. An attempt is made here to summarize some of these breakthroughs and outline their significance in broader perspectives. In the present study, a total of 12 samples were collected from four types of water sources for isolation of Vibrio spp. Water samples are enrichment into alkaline peptone water then inoculate into culture media such as Nutrient agar, MacConkey, and Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar medium. After incubation of TCBS plates for 24h at 37°C yellow and green colonies are screened out for biochemical identification. No-sucrose fermenting Vibrio parahaemolyticus, Vibrio mimicus, and Vibrio vulnificus show green colonies and sucrose fermenting Vibrio cholerae and Vibrio alginolyticus show yellow colonies on TCBS. To identify the Vibrio spp isolates biochemical test was carried out and typical Vibrio spp give a positive result. The majority of Vibrio spp are avirulent, but certain strains may be sporadically human pathogenic. The antibiotic resistance studies showed that among the 9 isolates were resistant against Erythromycin, Penicillin, Cephalexin, Vancomycin and 4 isolates resistant against Tetracycline. To expand current knowledge of the occurrence, ecological niche and persistence of potential human pathogenic Vibrio spp in aquatic environments, occurrence, and laboratory studies were performed.
Highlights
Water is the necessity for life and an adequate, safe and accessible supply is of paramount importance
3.1 Separation and study of cultural characters: In the present study, 12 water samples were collected from different sources such as tap water, pond water, river water and sewage water for isolation and identification of Vibrio spp gram negative bacteria
Before enrichment samples were inoculated into culture media such as Nutrient agar, MacConkeys and Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar used for separation of Vibrio spp
Summary
Water is the necessity for life and an adequate, safe and accessible supply is of paramount importance. Life is possible on earth due to the presence of water. The impact of water on health is associated with unwholesome sources contaminated with faces. The quality of drinking water is closely associated with human health and providing safe drinking water is a major public health priority. It is the dispersion medium for all biochemical reactions of the living process and takes part in many of these reactions.
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