Antibiotic nitrofurazone drives the functional dynamics of periphytic protozoan fauna in marine environments

Abstract

The use of functional traits of a community as a method to measure its functional dynamics in response to environmental change has gained attention because trait-based approaches offer systematic opportunities to understand the interactions between species diversity and ecosystem function. However, the relationship between functional traits of periphytic protozoa and contamination of aquatic habitats with antibiotics is poorly understood. In this study, we investigated the influence of the antibiotic nitrofurazone on functional traits of marine periphytic protozoan fauna. For this purpose, the protozoan assemblages were collected from coastal waters of the Yellow Sea at Qingdao, northern China, during four seasons of a one-year cycle using glass microscope slides as artificial substrates. The test protozoan communities were then exposed to various treatments of nitrofurazone in laboratory bioassay experiments. Our results demonstrated that the modalities of the functional traits of protozoan communities were generally driven by nitrofurazone toxicity. Briefly, R-mode linked to Q-mode (RLQ) and fourth-corner analyses revealed strong positive correlations between functional traits and nitrofurazone treatments. Trait syndromes in terms of body length, width, weight, height, and size to volume ratios were significantly influenced by nitrofurazone exposure. In particular, small and medium body size species of different feeding types, i.e., algivores, bacterivores, raptors or non-selectives, were more sensitive than other protozoan species to higher concentrations of nitrofurazone. Our findings demonstrate that antibiotic toxicity is likely to affect periphytic protozoan community function, shape the functional processes, and induce toxic responses in the community. The findings of this study suggest that periphytic protozoan communities and their functional traits are suitable bioindicators for evaluating the ecotoxicity of nitrofurazone in marine environments.