Abstract
Diabetic foot complications are very common and represent a serious health problem in Mexico because of their high frequency, high costs and difficulties in handling. The treatment of choice to inhibit bacteria related to diabetic foot ulcer consists mainly of the use of cefotaxime however the problem with this treatment (antibiotics) is not always effective due to the pathophysiological condition of the patient, together with the resistance bacteria develop to the drugs. OMS has suggested the use of probiotics for research directed to the development of microbial interference therapies. This project used the Lyophilized conditioned medium with probiotics, extracellulars of probiotics, because there are reports in which wound healing in mice is observed employing probiotics. The objective of this study was to evaluate the biological activity of cefotaxime, clindamycin and thelyophilized conditioned media Lactobacillus acidophilus (LCMLa) on bacterias isolated from diabetic foot ulcer, this bioassay was performed by the turbidimetric method. The macroscopic analysis of the colonies was carried out and the morphological analysis of the bacteria was carried out using the atomic force microscope; in addition, the type of Gram and oxygen requirements for its growth were determined. From the diabetic foot ulcers, three strains were isolated, of which strain 1 and 3 whose morphology corresponds to a bacillus, was susceptible to cefotaxime and to the lyophilized conditioned medium of L. acidophilus. The potential of microbial interference that exhibits L. acidophilus on bacteria related to diabetic foot ulcer is demonstrated.
Highlights
Diabetes is an assimilation of carbohydrates disorder, carbohydrates are all possible forms of simple and complex sugars in our food chain
The objective of this study was to evaluate the biological activity of cefotaxime, clindamycin and thelyophilized conditioned media Lactobacillus acidophilus (LCMLa) on bacterias isolated from diabetic foot ulcer, this bioassay was performed by the turbidimetric method
Strains isolated from diabetic foot ulcer was identified as 1, 2 and 3, in Table 2 shows the results of the microscopic analysis and macroscopic description and requirement of oxygen
Summary
Diabetes is an assimilation of carbohydrates disorder, carbohydrates are all possible forms of simple and complex sugars in our food chain. In diabetes the production of enough insulin in the pancreas usually occurs, but at the cellular level sensitivity to the effect of insulin is low. It is known as a relative insulin deficiency. In Mexico, diabetes has become the leading cause of death by contributing 12% of total deaths. More than 80% of diabetes deaths occur in countries of low and middle income. Half of those deaths are in people under 70, and 55% women [1]
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