Antibiogram signatures of Vibrio species recovered from surface waters in South Western districts of Uganda: Implications for environmental pollution and infection control

Abstract

Reports of vibriosis and other related gastrointestinal infections have remained a recurring concern in the diverse global continent. The safety of drinking surface water and associated environmental pollution has remained a public health concern in limited resource sittings. Seen in this light, we report the antibiogram signatures of Vibrio species recovered from surface waters in the South-Western districts of Uganda. Surface water samples were collected for four months for Vibrio species isolation in four districts (Bushenyi-B, Mitooma-M, Rubirizi-R, and Sheema-S) using bacteria culture procedures, disc diffusion and Polymerase Chain Reaction (PCR) technique. Isolates were characterised, and the antibiotic fingerprints were determined using PCR and nine selected antibiotics in routine use. A total of 392 Vibrio species were confirmed from the various districts (B: 172, M: 79. R: 60, S: 81), with 163 (94.77%) resistant to colistin (polymixin), 145 (84.3%) resistant to cefotaxime, 127 (73.84%) resistant to azithromycin, and 33 (19.19%) resistant to chloramphenicol among Bushenyi isolates. A similar high resistance to fluoroquinolones and carbapenem antibiotics were also recorded in other districts of the study area. A complete multiple antibiotic resistance phenotype ((M)ARPs) to the applied antibiotics (A-CTX-CXM-MEM-ATH-K-TM-C-PB-NI-CIP-NA) were also recorded among some isolates, which produced multiple antibiotic resistance indexes of 1, suggesting a high-risk source of contamination due to the usage of several antibiotics. The PCR reports also confirm ampC gene {20 (10.9%)}, beta-lactamase TEM gene (blaTEM2), {30 (10%)} and dihydropteroate synthase type-1 and 11 gene (sul 1 & 11) {16 (8%)}. The results present an implicated environmental pollution problem and a potential concern to public health, therefore there is the need for control of such infectious bacteria and environmental pollution monitoring. Hence, it is recommended various approaches crucial to monitoring of emerging trends in drug resistance at the local and international levels.