Abstract
This research aimed to study the antibacterial activity and Antibiotic effect of hot aqueous extract of leaves of Ziziphus spina Christy (Sidr),against clinical isolate of Staphylococcus wernari. four isolates were initially diagnosed as Staphylococci bacteria, then one isolation was chosen depending on its sensitivity and resistance to several different types of antibiotics. The VITEK-2 compact system (ID and AST) and PCR were used to confirm the type of staphylococcal isolates . The results showed that one isolate was due to Staphylococcus wernari. the ability of isolate to form biofilm was tested using the Microtiter plate method (96 wells). The results indicated that the extracted isolate was able to produce a biofilm. Where there was a clear effect of moxifloxacin and penicillin G with (MIC), (Sub-MIC) and (Sub-MIC) in inhibiting the growth of bacteria, A significant (P < 0.001).Synergistic effect between Penicillin G, Moxifloxacin with (MIC), (Sub-MIC) and (Sub-MIC) were tested with hot aqueous extract of Ziziphus spina christi (Sidr) leaves with 50 mg/mL. The results showed a high synergy between two types of antibiotics and plant extracts.
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