Antibiofilm Activity of Parabiotic Reuterin on Acrylic Resin Plates

Abstract

Denture stomatitis is the most common debilitating problem among denture users worldwide. An alternative treatment could be natural probiotics. Probiotic Lactobacillus reuteri produces an antimicrobial substance called reuterin. The aim of this study is to evaluate effectiveness of parabiotic reuterin towards Candida albicans and Staphylococcus aureus biofilms on acrylic resin plates. The minimum inhibitory concentrations (MICs) of reuterin towards C. albicans and S. aureus were evaluated. Thereafter, standard preventive and therapeutic assays were performed on biofilms grown on acrylic denture plates. The effect of reuterin on biofilms was quantified with crystal violet staining, colony counting, and qPCR. The experimental data were analyzed by appropriate statistical tests. The MICs of reuterin towards S. aureus and C. albicans were 100µg/mL and 25µg/mL, respectively (p<0.05). Interestingly, reuterin exhibited antibiofilm activity against S. aureus and C. albicans at 100 µg/mL and eradicated the biofilms by 24 h incubation (p<0.05). Moreover, reuterin was effective against monospecies and mixed-species biofilms on acrylic plates. This pilot study showed that reuterin has an antibiofilm activity against C.albicans and S.aureus biofilms by reducing biofilms’ number as much as 83% and 94.5 % respectively, in the denture acrylic plate model Hence, result will provide a basis for developing reuterin as a probiotic-based natural solution for denture stomatitis in the future.