Antibiofilm activity of klebocin crude extract against some species of Enterobacteriaceae

Abstract

Bacteriocins are antibacterial proteins created by bacteria and its effective against other strains of bacteria which are closely linked to the producing strains and a number of species from the same family. The aim of current study was to evaluate the activity of bacteriocin that extracted from clinical isolates of Klebsiella pneumonia against different species of enterobacteriaceae in both planktonic and biofilm state. The antibacterial activity of bacteriocins (klebocins) from Klebsiella pneumoniae isolates towards diverse pathogenic species from enterobacteriaceae (by well assay method) was demonstrated , however the antibiofilm activity against some species of enterobacteriaceae was studied also by Tissue culture plate method (TCP) in first and last stages of biofilm formation .The results revealed that klebocin with different concentrations was efficient against different pathogenic species by producing different inhibition zones. Results also showed that klebocins had a wide antibiofilm activity on some pathogenic species of Gram-negative bacteria .In addition to these finding it was observed that the antibiofilm activity of klebocin on premature biofilm of Klebsiella was higher than its effect towards other pathogenic species, while it was affected on mature biofilm of other bacterial species as well as Klebsiella.