ANTIBIOFILM ACTIVITY AND STABILITY ASSAY OF PIGMENT FROM MARINE BACTERIA Bizionia paragorgiae

Ignes Nathania,Diana Elizabeth Waturangi

doi:10.36547/be.330

Abstract

Biofilms are communities that contain one or multiple microorganisms that enclosed in extracellular polymeric substance (EPS) matrix Biofilm formation is one of important survival skills for bacteria to be able to grow in all surfaces, such as living tissues, medical devices, industrial water piping, shipping, and natural aquatic system. It is recognized as the important virulence factor and protection for bacteria from environment influences. Bizionia paragorgiae, which produced orange pigment, was examined in this research. In this research, antibacterial, antibiofilm activity, and stability assay were examined. There was no antibacterial activity indicated from this study against all tested Gram-negative and Gram-positive bacteria. Based on antibiofilm activity against four pathogenic bacteria, both destruction and inhibition assay reduced biofilm formation by up to 30-70%. According to the result revealed from stability assay, the pigment was more stable at its natural pH 5.83, at 25 °C, and stored in dark condition.

Highlights

Biofilm is an important virulence factor, both for opportunistic and true pathogens
Materials used in this study were crude extract of pigment from isolate Bizionia paragorgiae; Marine Agar, Marine Broth (Difco, Franklin Lakes, New Jersey); Brain Heart Infusion, Eosin Methylene Blue Agar, Yeast Extract, Tryptone, Bacteriological Agar (Oxoid, Basingstoke, Hampshire); NaCl, D-glucose, Methanol Absolute, Ethanol Absolute, and Crystal Violet (Merck, Darmstadt, Hesse)
RESULTS Antibiofilm activity In order to evaluate whether the pigment was able to reduce biofilm formation, antibiofilm assay was performed against four pathogenic bacteria (S. aureus, Escherichia coli (EHEC), Enterotoxigenic Escherichia coli (ETEC), and Enteropathogenic Escherichia coli (EPEC))

Summary

Introduction

Biofilm is an important virulence factor, both for opportunistic and true pathogens. Bacterial biofilms can develop on many abiotic surfaces, such as plastic, glass, and metal, and on biotic surfaces, such as human, animal, and plants. Because the polymer extracellular matrix acts as the shield, the bacterial growth in biofilm is more resistant to antimicrobial agents and chemical biocides, leading to much effort for researches to discover new compound that has antibiofilm and antibacterial performance (Thenmozhi et al, 2009; Bernbom et al, 2011). Pigments from marine bacteria are known to have antibacterial and antibiofilm activity. Marine bacteria are great potential source of bacterial pigment, since they are abundant in the ocean that covers 70% of the Earth’s surface. With the vast sea area and plentiful marine microorganisms, Indonesia offers a promising potential for discovering new marine bacterial pigment. The objectives of this study were to discover the antibiofilm activity of marine bacterial pigment isolate Bizionia paragorgiae and determine the stability of the pigment in various pH, temperature, and in the presence or absence of light

Objectives

Methods

Results

Discussion

Conclusion