Abstract

Silver nanoparticles can be prepared by using a microorganism. The objectives of the study were to determine the ability of Fusarium oxysporum BNT-02 in reducing silver nitrate solution to silver nanoparticles, to evaluate the effect of variations in treatment of textile materials toward antibacterial activity of textile materials impregnated with silver nanoparticles on Staphylococcus aureus ATCC 25924 and Escherichia coli ATCC 35218. In this study, silver nanoparticle were obtained by reducing the AgNO3 solution by F.oxysporum BNT-02. Coating of silver nanoparticles on textile materials was carried out by inserting sterile textile material into silver nanoparticle and shaking then drying. The textile material modified with silver nanoparticles was characterized for antibacterial activity against S.aureus ATCC 25924 and E.coli ATCC 35218. The inhibition zone to determine antibacterial activity was observed every 6 hours for 48 hours of incubation time. The inhibition zone data was analyzed using ANCOVA and t-test. The results showed that F.oxysporum BNT-02 can be used as a reducing agent for producing silver nanoparticles. The textile material coated with silver nanoparticles had antibacterial properties against S. aureus ATCC 25924 and E. coli ATCC 35218. Antibacterial activity of textile materials coated with silver nanoparticles on S.aureus ATCC 25924 showed a larger inhibition zone than E.coli ATCC 35218.

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