Antibacterial Test from Ekstract of Kirinyuh Leaves to Inhibit Vibrio sp. Bacteria Growth which Caused Ice-ice Diseases in Seaweed (Eucheuma cottoni)

Abstract

Indonesia is the largest archipelagic country so it has enormous coastal potential as well. One of the coastal potential that has great benefits for the community is seaweed cultivation. Production of seaweed cultivation in NTB province in 2012 reaches 657,700 tons and increase every year. But in fact, the development of seaweed cultivation business have several obstacles, one of them is the presence of ice-ice disease that infects the seaweed Eucheuma cottoni species. This ice-ice disease causes a decrease in the quality and quantity of seaweed causing crop failure. This ice-ice disease attacks the thallus in the seaweed and causes the seaweed thallus to become brittle and breaks easily due to the lysis of epidermal cells and chloroplasts. The ice-ice disease is caused by a bacterial infection of Vibrio sp. which occurs when seaweed become stress due to adverse environmental conditions such as rising temperatures and salinity. The ice-ice disease can be threat using antibacterial compounds which found in plants such as tannins, flavonoids, and saponins. One of the plants that contain antibacterial is kirinyuh (Chromolaena ordorata). The antibacterial content on kirinuyuh leaves is extracted using a simple maceration method with aquadest as a solvent, and yields of extraction was diluted into concentrations of 20%, 40%, 60%, 80%, and 100%. Leaf extract of kirinyuh with variation of concentration was tested in vitro condition by using diffusion agar method with paper disk on solid NB media which has been overgrown with Vibrio sp. The result of in vitro test using diffusion agar method with paper disk was obtained that kirinyuh extract is able to inhibit the growth of Vibrio sp. bacteria with the widest inhibit zone is 10.315 mm at 100% concentration.