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Abstract
In the present report, Lemon juice (bio-extract) extract was efficaciously used for the synthesis of bio-functionalized silver nanoparticles (Ag-1, Ag-2 & Ag-3 NPs) and decorated with carbon material obtained from mustard oil. The morphology, size, crystal structure, formation and interaction were studied by means of innumerable analytical methods including scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FT-IR) spectroscopy. The XRD results approve the formation of AgNPs with face-centered cubic (fcc) lattice. The XRD results also exhibit some unassigned peaks which might be due to the presence of bio-organic materials on the surface of AgNPs. The bands observed from the IR spectra showing the involvement of biomolecules onto the surface of silver nanoparticles. Mostly citric acid plays a major role in bio-reduction, capping agent, and stabilization of silver ions. We attained maximal inhibition zone (2.10 ± 0.05 and 2.03 ± 0.027) counter to gram-negative bacteria K. pneumoniae and P. bacilli with Ag-3 respectively, but lowest inhibition zone (1.27 ± 0.22) contrary to S. aureus as a gram-positive bacteria with Ag-2.
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