Abstract
Transgenic potato plants have been produced that express and secrete the foreign bacteriophage T4 lysozyme. The chimeric barley a-amylase signal peptide — T4 lysozyme gene is driven by the CaMV 35S promoter. Low level expression can be detected at mRNA and protein level. Subcellular localization analysis demonstrates secretion of the foreign protein to the intercellular spaces. Biological in vitro and greenhouse experiments demonstrate that tuber maceration following inoculation with Erwinia carotovora atroseptica in transgenic tissue is significantly reduced in comparison to control tissues. Moreover, explants from inoculated transgenic tuber pieces show sprouting and growth without development of disease symptoms. Expression and secretion of the foreign lysozyme obviously leads to a reduced susceptibility of the transgenic potato plants towards the phytopathogenic bacterium.
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