Abstract
The antibacterial activity of honey is mainly credited to its acidity, osmolarity and enzymatic generation of hydrogen peroxide via glucose oxidase. Additional honey components, such as aromatic acids or phenolic compounds, also contribute to the overall antibacterial activity. The level of antibacterial activities found in honey varies with different types of honey, due to mainly the composition, percentage as well as the nature of the sugars present in the honey. This study aimed to evaluate the antibacterial activity of four types of honey, namely Tualang honey (TH1), Tualang honey (TH2), Acacia honey (AH) and Yemeni Sumur honey (YSH). Nine bacterial strains were used. Disc diffusion, well diffusion, minimum inhibitory concentration (MIC), Minimum bactericidal concentration (MBC), and time-kill methods were performed to reveal the antibacterial potential of the selected honey. The MIC values ranged between 12.5 to 50% for both TH1 and YSH while for TH2, and AH it ranged between 25 to 50%. For MBC, it ranged from 25 to 50%. The time-kill in TH1 Staphylococcus aureus (food isolate) showed total inhibition at 6 hrs in 2 X MIC, and for Staphylococcus aureus ATCC 29737 was 3.84 log CFU/g at the 6 hrs. Physicochemical quality of honey resulted as follows: the pH of the honey samples was acidic in nature ranging between 3.69 to 3.94, and the aw of the honey samples were between 0.53 to 0.69. For colour analysis, YSH was observed to has the maximum lightness and yellowness, and TH1 has the maximum redness. While, AH had a minimum lightness, redness, and yellowness.
Highlights
Honey is characterised as a vicious, aromatic and sweet food substance that many people around the world enjoy its consumption
The results reported were the mean of five determinations (Shafiee et al, 2014)
The diameter of the inhibition zone of the positive control was between 22.00 – 24.24 mm, which was higher than the 50% concentration of treatments
Summary
Honey is characterised as a vicious, aromatic and sweet food substance that many people around the world enjoy its consumption. Honey has been used in medical treatment as an antiseptic until the antibiotics were invented. The treatment of diseases has become more difficult than ever due to the emergence of antibiotic-resistant bacteria. Humans seek a natural alternative such as honey to treat diseases. Honey contains antibacterial compounds that are effective in killing or inhibiting a broad spectrum of bacteria (Boukraâ, 2013). Various studies have shown that honey is effective against a wide range of microorganisms, including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycinresistant enterococci (VRE)
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