Abstract
Background: Pyrrosia longifolia is an epiphytic plant found in tropical forests. This plant is known to have antioxidant, anti-diabetic, anti-inflammatory, and antibacterial properties and is widely used in traditional medicine. Pyrrosia longifolia is one of the species used in traditional medicine, but no evidence of antibacterial activity has been found. Objective: The purpose of this study is to determine the antibacterial activity of various P. longifolia extracts. Methods: The plant was macerated with methanol and separated based on its polarity. The well diffusion method was used to determine the plant’s antibacterial activity through the inhibition zones - minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of bacterial growth against seven bacteria pathogens. Results: The antibacterial activity of the extracts showed various inhibitions of the bacteria tested. Dichloromethane and ethyl acetate extracts showed intermediate inhibitory activity on Bacillus subtilis ATCC 19659, B. cereus ATCC 10876, Salmonella typhimurium ATCC 142028, and Vibrio parahaemolyticus ATCC 17802. Conclusion: The extract of P. longifolia is susceptible to the test bacteria, and additional testing is necessary to determine its antibacterial activity.
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