Abstract
Secretions aseptically collected from larvae or maggots of the green bottle fly Lucilia sericata (Meigen) (Diptera: Calliphoridae) exhibit antimicrobial activity along with other activities beneficial for wound healing. With the rise of multidrug-resistant bacteria. New approaches for identifying the active compounds responsible for the antimicrobial activity were examined. Five different pathogenic bacterial strains were used in this study, Three of them are gram positive bacteria, namely: Staphylococcus aureus, streptococcus sp, and proteus sp and two of them are gram negative bacteria, namely: E. coli. and Salmonella typhi. Considering the activity against organisms typically associated with clinical infection, may be a source of novel antibiotic-like compounds that may be used for infection control. Therefore, the aim of this study was to use a novel approach to investigate the output of secreted proteins from the maggots under conditions mimicking clinical treatments.Results revealed that the secretions aseptically markedly inhibit the growth of both gram-positive and gram-negative bacteria.
Highlights
Maggots of the blowfly Lucilia sericata have been successfully used as a debridement agent for chronic and infected wounds through history
We make test on different bacteria strains by ES of larvae that fed on liver, meat and beef burger for 24h, 48h and 72h we obtained on the ES of larvae that fed on meat is higher effect and the inhibition zone stop growth after 24h for three types of ES of larvae that fed on liver, meat and beef burger
According to results that we obtained when were the larvae reared on liver, meat and beef burger diets the meat food was a good media for the duration and the development of stages for this fly, and when we make test on different bacteria strains by ES of larvae that fed on liver, meat and beef burger for 24h, 48h and 72h found the ES of larvae that fed on meat is higher effect and the inhibition zone stop after 24h for three types of ES of larvae that fed on liver, meat and beef.( Tables 1, 2, 3) and Figs. (1, 2, 3 and 4) So that we measured inhibition zones every 4h for 24 hours for the ES of larvae that fed on meat
Summary
Maggots of the blowfly Lucilia sericata have been successfully used as a debridement agent for chronic and infected wounds through history. Due to the appearance of antibiotic resistance and increasing problems with chronic wounds worldwide, Seeking novel antibacterial substances and antibiotic combination therapy are the strategic options to overcome the, maggot debridement resistant MDR organisms (Jaklic et al, 2008, Steenvoorde and Jukema 2004). Such initiative has greatly driven the search for new antimicrobial agents with novel mechanisms of action that are broadly effective and less likely to induce antimicrobial resistance. These drugs will be very important, for the treatment of immune compromised patients (Guardabassi and Kruse 2003, Hujer et al, 2004)
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