Antibacterial Properties of Biodegradable Silver Nanoparticle Foils Based on Various Strains of Pathogenic Bacteria Isolated from the Oral Cavity of Cats, Dogs and Horses.

Miłosz Rutkowski,Lidia Krzemińska-Fiedorowicz,Aleksandra Suder,Marek Tischner,Gohar Khachatryan,Julia Kabacińska,Anna Lenart-Boroń,Klaudia Kulik

doi:10.3390/ma15031269

Miłosz Rutkowski, Lidia Krzemińska-Fiedorowicz + Show 6 more

Open Access

https://doi.org/10.3390/ma15031269

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Journal: Materials	Publication Date: Feb 8, 2022
Citations: 6	License type: CC BY 4.0

Affiliation: University of Agriculture in Krakow

Abstract

Frequent occurrence of microbial resistance to biocides makes it necessary to find alternative antimicrobial substances for modern veterinary medicine. The aim of this study was to obtain biodegradable silver nanoparticle-containing (AgNPs) foils synthesized using non-toxic chemicals and evaluation of their activity against bacterial pathogens isolated from oral cavities of cats, dogs and horses. Silver nanoparticle foils were synthesized using sodium alginate, and glucose, maltose and xylose were used as reducing agents. The sizes of AgNPs differed depending on the reducing agent used (xylose < maltose < glucose). Foil without silver nanoparticles was used as control. Bacterial strains were isolated from cats, dogs and horses by swabbing their oral cavities. Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and extended-spectrum beta-lactamase (ESBL) producing E. coli were isolated on selective chromogenic microbiological media. The bactericidal effect of AgNPs foils obtained using non-toxic chemical compounds against E. coli, ESBL, S. aureus and MRSA isolated from oral cavities of selected animals was confirmed in this study. No statistically significant differences were observed between the foils obtained with different reducing agents. Therefore, all types of examined foils proved to be effective against the isolated bacteria.

Highlights

Nanotechnology is among the modern fields of science that find their wide application in human and veterinary medicine, agriculture, food and feed production, cosmetic industry, pharmacy, heritage preservation against microbial biodeterioration, textile industry and optics [1,2,3,4]
When maltose was used as a reducer, we observed an increase of the size of the nanoparticles and change in the shape of nanocrystals
The conducted studies confirmed that the oral cavities of animals, such as cats, dogs and horses, are inhabited by bacterial pathogens, such as methicillin-resistant Staphylococcus aureus (MRSA) and extended spectrum beta-lactamase producing E. coli (ESBL), as well as by opportunistic pathogens, such as methicillin-susceptible S. aureus and non-extended-spectrum beta-lactamase (ESBL) producing E. coli

Summary

Introduction

Nanotechnology is among the modern fields of science that find their wide application in human and veterinary medicine, agriculture, food and feed production, cosmetic industry, pharmacy, heritage preservation against microbial biodeterioration, textile industry and optics [1,2,3,4]. It is the chemical method that involves the reduction of metal salts with the use of numerous reducing substances that allows for obtaining various shapes of nanoparticles [5,6,7]. Due to various shapes of the adopted structures, silver nanoparticles exhibit numerous biological effects. One of the most important properties of metal nanoparticles is their antimicrobial activity. It results from a number of actions, leading to the destruction of their biological structures and limiting the microbial growth. The mechanisms of antibacterial action of silver nanoparticles (AgNPs) include: disruption of cell wall and cytoplasmic membrane perforation and denaturation, denaturation of ribosomes, interruption of ATP production, cytoplasmic membrane disruption by reactive oxygen species and interference with DNA replication [8,9,10,11,12]

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