Antibacterial Potentiality of Red Sandalwood Callus against Pathogenic Isolates of Aeromonas and Pseudomonas

Abstract

The present study is based on the in vitro production of callus, its long period maintenance and antibacterial potentiality. Callus induction and formation occurred by full strength MS media in combination with various concentration (0.5mg/l, 1.0mg/l, 1.5mg/l, 2.0mg/l) of 2, 4-D effective in combination with constant concentration of BA (2.5mg/l) and NAA (0.5mg/ml). The leaf calli were sub cultured and transferred to MS media with various hormonal combinations which were different from callus induction media. Internodes were also subjected to induce callus and callus formation resulted from MS media in combination with Kinetin (0.5mg/l, 1.0mg/l, and 1.5mg/l), BA (1.0mg/ml, 2.5mg/ml), NAA (1.0mg/l, 1.5mg/l, 2.0mg/l and 2.5mg/l) without adding 2, 4 D and another combination of 0.5mg/l 2, 4 D and 2.5 mg/l BA as well as 0.5mg/l NAA . The sustainability rate of total leaf callus for four month was 83.33% and that for inter node callus was 56.65%. Ten strains of Aeromonas and Pseudomonas were used to antibacterial screening. The crude methanolic extract showed antibacterial activity against four strains of Aeromonas (E14, Cok2/2, Cok2/3, Cok2/4) and one strain of Pseudomonas (P2F4). Ethanolic extract absorbed filter paper discs showed very low efficiency of inhibition to two strains of Aeromonas E17, Cok2/4 and one strain of Pseudomonas P2F4. Crude callus extract was effective against the growth of three strains of Aeromonas (E14, E17 and Cok2/4). It prohibited the growth of Pseudomonas Cla1b10 (avg. 10.71mm). This result implies the potentiality of presence of active compounds or secondary metabolites that have the antibacterial features in Red sandalwood callus.