Abstract
Abstract To solve the bio-inertness of widely used polypropylene (PP) mesh for treating pelvic floor dysfunction (PFD), a novel strategy of incorporation with elastin gene-transfected bone marrow stem cells (BMSCs) and antibacteria drug-loaded polylactic acid (PLA) nanofibrous mat covering layer was proposed to overcome the limitation of the pristine PP mesh. Then, a series of physicochemical and in vitro experiments were applied to investigate the improvement of the as-prepared material. The elastin protein expression was proved to be upregulated without obvious cytotoxicity influence after the gene transfection and also improved the cell migration rate. In addition, the antibacteria drug-loaded PLA nanofibrous mat on the PP mesh could efficiently inhibit bacteria and showed no significant impact on cell adhesion and proliferation. Thus, we believe that the incorporation of the elastin gene-transfected BMSCs and nanofiber-coated PP mesh would be a potential candidate in the application of female PFD.
Highlights
Nanotechnology was applied in several research directions, and materials with different components and nanostructures were developed to fulfill different requirements [1–5]
The transfected bone marrow stem cells (BMSCs) were cultured to fully cover the plate and extracted with RIPA buffer, the supernatant was centrifuged at 12,000 rpm for 15 min, and the total protein content of which was evaluated by Coomassie Brilliant blue solution
The results indicated that the ciprofloxacin hydrochloride (CIP) could successfully release from polylactic acid (PLA) nanofibers and displayed a strong antibacteria activity in a relative low concentration
Summary
Nanotechnology was applied in several research directions, and materials with different components and nanostructures were developed to fulfill different requirements [1–5]. The major factors of this phenomenon include the tissue compatibility to the mesh and the fiber un-degradation caused by inflammation around the mesh; a second operation should be undertaken to address this problem [25] To avoid this after mesh implanting, the materials have to be modified and improved. Due to the chemical inert surface and large holes of PP mesh, the genetransfected cells could not be loaded and implanted together to the injured tissue; certain method should be developed to deal with this problem. After measuring the expression ability of elastin gene-transfected BMSCs, nanofibrous mat of biocompatible polymer was initially coated on PP mesh as a support of cell adhering (Figure 1). To prevent probable inflammation during implantation, antibacteria component was loaded in the nanofibrous mat [44], the antibacterial efficiency and biocompatibility were investigated to estimate the viability of the applied materials [45,46]
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