Antibacterial efficacy of lactic acid bacteria and bacteriocin isolated from Dadih’s against Staphylococcus aureus

Usman Pato,Emma Riftyan,Dewi Fortuna Ayu,Mosaad Attia Abdel-Wahhab,Jeska Aryaningsih Feruni,Mimi Sri Wahyuni,Nia Naidya Jonnaidi

doi:10.1590/fst.27121

Abstract

Bacteriocin, a peptide produced by lactic acid bacteria (LAB) widely used as a natural and safe preservative in food products. This work aimed to evaluate the characterization of bacteriocin from dadih’s LAB and to study its antimicrobial activity against Staphylococcus aureus. Supernatant from 12 LBA strains could inhibit S. aureus growth with different inhibition zones. Only nine strains showed inhibition zones of > 3.5 mm and were further evaluated for their antibacterial compounds. Three strains had antimicrobial activity derived from organic acids, especially lactic acid, and six strains had antimicrobial compounds in the form of bacteriocin. Bacteriocin strain R-55 showed the highest activity against S. aureus, with an average inhibition zone of 8.43 mm. The molecular weight of the purified isolated bacteriocin from the R-55 strain was 14.4 kDa. Bacteriocin obtained from St. faecalis subsp. liquefaciens R-55 is a promising natural preservative to prevent the growth of S. aureus as foodborne pathogens.

Highlights

Staphylococcus aureus is the major pathogenic bacteria that infect humans and animals, resulting in several clinical manifestations of the pathogen, including the infection of superficial skin and soft tissue, sepsis, pneumonia, and endocarditis (Tong et al, 2015)
These results indicated the secretion of antibacterial compounds into the extracellular environment during lactic acid bacteria (LAB) growth, as shown by the clear zone
The difference in antimicrobial activity of the isolated 12 strains of LAB is likely due to differences in the amount of lactic acid or the type of bacteriocin produced by each LAB

Summary

Introduction

Staphylococcus aureus is the major pathogenic bacteria that infect humans and animals, resulting in several clinical manifestations of the pathogen, including the infection of superficial skin and soft tissue, sepsis, pneumonia, and endocarditis (Tong et al, 2015). These pathogenic bacteria are prone to be resistant to different antibiotics. MRSA can epidemically spread in livestock and hospitals, societies (Ho et al, 2012; Vandendriessche et al, 2014). The risk of transmission of MRAS from animals to human attracted public health concern since the observation of the livestock-associated MRSA from pig belonging to LA-MRSA CC398 (Clonal Complex 398) was reported in Netherland early in the twentieth century (van Loo et al, 2007; Garcia-Graells et al, 2012)

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