Abstract
Aim: To evaluate the efficacy of natural agents for disinfection of root canal system, such as the effect of Azadirachta indica (neem), thyme oil, and 2% chlorhexidine gluconate (CHX) on multispecies biofilm of most commonly found pathogens such as Streptococcus mutans and Enterococcus faecalis in the field of endodontics. Materials and Methods: To visualize the antibacterial effectualness of the methanolic extracts of the medicated plants and CHX, an in vitro dentin model was used. A polymicrobic biofilm was grown on human extracted teeth for 21 days. Microbial load in the form of colony-forming units per milliliter (CFU/mL) after the antimicrobial treatment was calculated. Data were statistically analyzed using analysis of variance and Bonferroni post hoc tests. Results: Plant extracts showed remarkable antibacterial efficacy along with 2% CHX compared with that of control. A significant statistical difference was found with 2% CHX. Conclusion: The methanolic extract of A. indica, thyme oil, and CHX has shown considerable antimicrobial activity against polymicrobic dentinal biofilm of S. mutans and E. faecalis.
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