Abstract
Objective: This study aimed to compare the antibacterial efficacy of green tea extract to chlorhexidine 2% against Enterococcus faecalis biofilm usingreal-time polymerase chain reaction (RT-PCR).Methods: E. faecalis strain ATCC 29212 colonies were collected from overnight cultures grown on brain–heart infusion agar (BHIA) and resuspendedat 108 CFU/ml according to a 0.5 McFarland standard. Aliquots of bacterial suspension (50 μl) were then inoculated onto sterile nitrocellulose filterdiscs place on BHIA and incubated aerobically at 37°C for 72 h. After incubation, the discs were removed, transferred into 10-ml phosphate-bufferedsaline (PBS) to loosen planktonic bacteria, and then incubated for 10 min at 37°C in 10 ml of 6% green tea extract, 2% chlorhexidine, or sterile PBS(control). Surviving E. faecalis cells were then quantified by RT-PCR.Results: Green tea extract reduced bacterial survival compared to control but was not as effective as chlorhexidine 2%.Conclusion: Green tea extract may help reduce oral E. faecalis biofilm. Moreover, specific antimicrobial compounds in green tea extract such asepigallocatechin-3-gallate should be tested as non-toxic alternatives to chlorhexidine.
Highlights
The success of root canal treatment is dependent on the effective elimination of microbes and irritants from the root canal system, which prevents reinfection and promotes healing
It would be advantageous to identify natural agents with strong antibacterial efficacy against E. faecalis but low toxicity against mammalian cells to enhance the biosafety of bacterial clearance during root canal treatment
While several studies have been conducted to test the antibacterial efficacy of green tea extract, none have directly compared its antibacterial effect to 2% chlorhexidine solution against E. faecalis bacteria in biofilm
Summary
The success of root canal treatment is dependent on the effective elimination of microbes and irritants from the root canal system, which prevents reinfection and promotes healing. Some bacterial species can survive even in prepared, irrigated, and obturated root canals, such as Enterococcus faecalis, which contributes to the persistence of periradicular lesions after root canal treatment. Many studies have examined the most effective way for removing E. faecalis from the root canal, and chlorhexidine has shown high efficacy [2,3,4,5]. It would be advantageous to identify natural agents with strong antibacterial efficacy against E. faecalis but low toxicity against mammalian cells to enhance the biosafety of bacterial clearance during root canal treatment. While several studies have been conducted to test the antibacterial efficacy of green tea extract, none have directly compared its antibacterial effect to 2% chlorhexidine solution against E. faecalis bacteria in biofilm
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