Abstract
In our current study, phytochemical analysis and antibacterial activity of leaf extracts of T. parthenium were assessed. Qualitative phytochemical screening was carried using standard procedures and quantitative analysis for estimation of total phenolic content (TPC) was determined by spectrophotometric method. The antibacterial activity was accomplished using disc diffusion assay, determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against two gram positives (Streptococcus pyogenes (ATCC 19615), Streptococcus anginosus) and two gram negatives (Pseudomonas aeruginosa (ATCC 27853) and Fusobacterium necrogenes) pathogenic bacteria. Preliminary phytochemical analysis of the plant extracts revealed the presence of phenolics, tannins, flavonoids, terpenoids, and cardiac glycosides. The methanol extract had highest total phenolic content (6.17 ± 0.19 mg/ml). The results also displayed that the methanol extract exhibited significant antibacterial activity against the tested pathogenic microorganisms, in particular against S. anginosus with inhibition zone of 15.33±0.33 mm. The lowest MIC (33.33 mg/ml) and MBC (41.66 g/ml) values were observed in methanol extract against S. anginosus and S. pyogenes respectively. The results indicated that crude leaf extracts of T. parthenium had a potent antibacterial activity suggesting that it could be valuable in treating pathogenic bacterial infections.
Highlights
Since time immemorial, plants have been used as source of remedies for the treatment of several ailments including infectious diseases by the people of all parts the world
The results indicated that crude leaf extracts of T. parthenium had a potent antibacterial activity suggesting that it could be valuable in treating pathogenic bacterial infections
Our study reported the preliminary phytochemical analysis, estimation of total phenolic content and antibacterial activity of T. parthenium leaves against four pathogenic bacteria such as S. anginosus, S. pyogenes, F. necrogenes and P. aeruginosa
Summary
Plants have been used as source of remedies for the treatment of several ailments including infectious diseases by the people of all parts the world. Side effects of practice of these drugs have amplified (Tsuruga, 2007) These motives have driven researchers to hunt for novel antimicrobial agents from plants substitute to synthesized chemical drugs and numerous studies were reported on the antibacterial activities of the medicinal plants (Sasikumar et al, 2007, Costa et al, 2017). Keeping this in mind, in the current investigation, T. parthenium (Asteraceae) was evaluated for its antibacterial activity against certain bacterial pathogens. The general goal of this current study is to analyse the phytochemical analysis and to assess antibacterial properties of various extracts of T. parthenium leaves against two gram positive and two gram negative bacterial pathogens
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