Antibacterial Effects of Chemical Constituents Isolated from the Roots of &amp;lt;i&amp;gt;Cordia myxa&amp;lt;/i&amp;gt; (Boraginaceae) on &amp;lt;i&amp;gt;Salmonella typhi&amp;lt;/i&amp;gt; (Enterobacteriaceae)

Dabole Bernard,Moussa Djaouda,Loura Benoit,Wakayansam Bouba Romeo,Abdou Tchoukoua,Meli Alain,Matcheme Mathieu

doi:10.11648/j.ajcbe.20210501.11

Antibacterial Effects of Chemical Constituents Isolated from the Roots of &lt;i&gt;Cordia myxa&lt;/i&gt; (Boraginaceae) on &lt;i&gt;Salmonella typhi&lt;/i&gt; (Enterobacteriaceae)

Dabole Bernard, Moussa Djaouda + Show 5 more

Open Access

https://doi.org/10.11648/j.ajcbe.20210501.11

Copy DOI

Abstract

Cordia myxa is a Cameroonian pharmacopoeia traditional plant. It belongs to the Boraginaceae family, which different parts are used in the treatment of various bacterial infections. The phytochemical screening carried out on the Cordia myxa species indicates the presence of alkaloids, triterpenes, steroids, carbohydrates, flavonoids and saponins. Our investigations on the roots of this plant allowed us to isolate two triterpenes from the ethyl acetate extract, which crystallize in the Hex/AcEt [2/8] solvent system; (23R) campesta-9 (11), 24 (30) diene-18,23-diol 3-O-α-rhamnopyranoside (compound 1) and alphitolic acid (compound 2). The isolation and characterization of these compounds were carried out respectively by silica gel chromatography and by NMR (1D, 2D) spectral analysis. Compound 1and 2 were tested on the in vitro growth of E.coli and Salmonella typhi using the Muller-Hinton solid medium disc method. The results revealed that these compounds have a dose-dependent antibacterial activity on Salmonella typhi. However, compound 2 had a better antibacterial potential on the Salmonella strain (18.6±0.6mm, 30mg/ml) with the MIC=0.25mg/ml than the compound 1 (14.5±0.4mm, 30mg/ml,) with the MIC=0.44mg/ml. Few classes of antibiotics have been tested, to look for those with the best results and compare the different doses of our compounds. The two compounds did not have any effect on E. coli.

Highlights

Typhoid fever is a serious foodborne illness caused by Salmonella typhi, a bacterium found in faecally contaminated water and food
Faced with the appearance of resistant forms of several bacteria to certain antibiotic, and the high cost of these products leading to the search for new active molecules with a broad spectrum of action has become a Dabole Bernard et al.: Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae) necessity [5]
The results obtained on the antibacterial activity of our compounds varied significantly depending on the concentration on Salmonella typhi

Summary

Introduction

Typhoid fever is a serious foodborne illness caused by Salmonella typhi, a bacterium found in faecally contaminated water and food. Faced with the appearance of resistant forms of several bacteria to certain antibiotic, and the high cost of these products leading to the search for new active molecules with a broad spectrum of action has become a Dabole Bernard et al.: Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae) necessity [5]. Cordia myxa is a plant from the tropical and subtropical region; it belongs to the Boraginaceae family. In Africa, the pulp of the fruit of Cordia myxa is used to treat diarrhea, dysentery, tuberculosis and ulcers [9]. In Cameroon, Cordia myxa is used to treat diarrhea, stomachaches, fever and urinary tract infections. The objective of this study is to isolate, characterize and evaluate the antimicrobial activity of secondary metabolites isolated from the root of Cordia myxa

Objectives

Results

Conclusion