Antibacterial Effects of Afzelin Isolated from Cornus macrophylla on Pseudomonas aeruginosa, A Leading Cause of Illness in Immunocompromised Individuals

Sang Lee,Young-Jin So,Moon Shin,Jongsung Lee,Jae Cho

doi:10.3390/molecules19033173

Sang Lee, Young-Jin So + Show 3 more

Open Access

https://doi.org/10.3390/molecules19033173

Copy DOI

Abstract

The crude ethyl acetate extract of the leaves of Cornus macrophylla showed antibacterial activity against Pseudomonas aeruginosa, a leading cause of illness in immunocompromised individuals. Bioactivity-guided separation led to the isolation of kaempferol 3-O-α-L-rhamnopyranoside (afzelin). The structure was determined based on evaluation of its spectroscopic (UV, MS, and NMR) data. The minimum inhibitory concentration (MIC) of afzelin against Pseudomonas aeruginosa was found to be 31 µg/mL. In addition, the results indicated that a hydroxyl group at C3 of the C-ring of the flavone skeleton and the rhamnose group may act as a negative factor and an enhancing factor, respectively, in the antibacterial activities of afzelin.

Highlights

IntroductionP. aeruginosa is part of a large group of free-living bacteria that are ubiquitous in the environment
P. aeruginosa is part of a large group of free-living bacteria that are ubiquitous in the environment.It can can cause a wide range of infections and is a leading cause of illness in immunocompromised individuals
The minimum inhibitory concentration (MIC) of gentamicin, a positive control, was found to be μg/mL. These results suggest that the hydroxyl group at C3 of the C-ring of the flavone skeleton and rhamnose group may be involved in antibacterial activity

Summary

Introduction

P. aeruginosa is part of a large group of free-living bacteria that are ubiquitous in the environment. It can can cause a wide range of infections and is a leading cause of illness in immunocompromised individuals. This organism can be a serious pathogen in hospitals [1]. Antibacterial resistance of P. aeruginosa and consumer mistrust of synthetic additives are increasing, creating a need for new antibiotics [4,5]. During our search for new antibacterial compounds from a taxonomically diverse plant collection, we found that Cornus macrophylla extract had a high antibacterial activity against. The antibacterial compound was evaluated to provide insight into the mode of action of this compound

Results and Discussion

Antimicrobial Activity of Isolated Compound against Pseudomonas aeruginosa

General Methods

Extraction and Isolation

Antimicrobial Test

Conclusions