Antibacterial effectiveness of different final irrigation activation techniques.

Abstract

Background and objective: To evaluate and compare the antibacterial effectiveness of ultrasonic, diode laser, XP endo finisher and finisher gentle file brush against Enterococcus faecalis in root canal dentin. Methods: Fifty-five single-rooted extracted mandibular premolars were decoronated to have a length of 12 mm, instrumented biomechanically with protaper Next up to size X3. After sterilization, five roots were selected as negative controls and the remaining teeth were inoculated with E. faecalis and incubated for 48 hr and at 37 ºC. The roots were then randomly divided into five groups (n=10), GI: Control group, GII: 5.25% NaOCl irrigation was activated by the ultrasonic system , GIII: By 980-nm diode laser , GIV: By Xp-endo finisher and GV : Activated by Finisher gentle file-brush. The antimicrobial efficacy was tested by collecting transfer fluid saline from the canals and counting the colony forming units (CFUs) of viable E. faecalis on agar plates. The statistical calculations were performed in JMP pro 14.3.0. using Analysis of Variance (ANOVA) followed by a Tukey test to define the differences between the tested groups. Result: The mean bacterial CFU were 10.6 X 10^7 for Finisher gentle file brush, 35.6×10^7 for Diode laser, 39.6×10^7 for ultrasonic and 42.3×10^7 for XP Endo Finisher. Finisher gentle file brush showed a significant reduction in the colony count compared to the other groups. Conclusion: None of the irrigation activation techniques removed the bacteria completely from the canal. The four tested irrigation activation techniques were shown to be effective in the disinfection of the E. faecalis-contaminated root canals. Finisher gentle file brush was the most effective against E. faecalis, while XP endo finisher was the least effective against E. faecalis. treatment against E. faecalis in the root canal. Keywords: Utrasonic, Diode laser, XP endo finisher, irrigation activation techniques