Abstract
Introduction: Virgin coconut oil (VCO) is a natural antibacterial agent with the ability to kill microorganisms whose cell membranes contain lipids, including Gram-positive and Gram-negative bacteria, such as Actinomyces sp. and Prevotella spp. No study on the antibacterial effects of VCO on the chromogenic bacteria that causes dental black stains.Objective: To analyze the effects of VCO, which administered in various concentrations to Actinomyces sp. and Prevotella sp.Methods: Actinomyces sp. and Prevotella sp. were isolated from the dental plaque of a child diagnosed with black stain. Each streak of bacteria was cultured on a selective medium and confirmed visually and through Gram staining. Each bacterial culture was exposed to VCO in concentrations of 12.5%, 25%, 50%, and 100%. Afterward, viability testing with a methyl-thiazolyl-tetrazolium assay was conducted, and the results were read using an Enzyme-linked immunosorbent assay reader.Results: The administration of 12.5% VCO reduced bacterial viability; however, 25% VCO significantly reduced the viability of Actinomyces sp. and 100% VCO significantly reduced the viability of Prevotella sp.Conclusion: Actinomyces sp. is more sensitive to VCO than Prevotella sp.
Highlights
Virgin coconut oil (VCO) is a natural antibacterial agent with the ability to kill microorganisms whose cell membranes contain lipids, including Gram-positive and Gram-negative bacteria, such as Actinomyces sp. and Prevotella spp
This study was conducted to identify the antibacterial effect of VCO on the bacterial viability of Actinomyces spp. and Prevotella spp., which cause dental black stain in children
The results of the bacterial culture were supported by the literature, which states that Actinomyces spp. and Prevotella spp. are primary chromogenic bacteria that play a role in the formation of dental black stain in children [1,11,16]
Summary
Virgin coconut oil (VCO) is a natural antibacterial agent with the ability to kill microorganisms whose cell membranes contain lipids, including Gram-positive and Gram-negative bacteria, such as Actinomyces sp. and Prevotella spp. No study on the antibacterial effects of VCO on the chromogenic bacteria that causes dental black stains. Dental stains found in children are categorized as black stains and are caused by anerobic chromogenic bacteria, namely, Actinomyces sp. Dental black stain has clinical characteristics, such as dark dots that form an interrupted line on two-thirds of the cervix of the tooth crown, following the contour of the gingiva, and adhering tenaciously to the dental surface [5,6]. Previous studies conducted in Jakarta in 2012 revealed that the ferrum content and amount of Actinomyces spp. is higher in the saliva of children with black stain than in children without black stain [7,8]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
