Abstract
Nigella sativa L. was allegedly to has antibacterial effect. The aim of this research is to investigate antibacterial effect of ethanolic extracts of 96% Nigella sativa L. from Indonesia toward Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus epidermidis. The Antibacterial activity of of Nigella sativa L toward Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus epidermidis using the well method. The 96% ethanolic extract of 12,5; 25;50 and 100% (mg/ml)inhibit growth of Staphylococcus aureus (with inhibition zone 11.06; 29.58; 28.22; 30.84 mm respectively) and Streptococcus epidermidis (20.90; 31.90; 29.93; 33.07 mm respectively). Ethyl acetate fraction of 96% ethanolic extract of Nigella sativa concentration of 6,25; 12,5; 25;50 and 100% (mg/ml) inhibit growth of Staphylococcus aureus (12.91; 15.06; 19.19; 37.48; 46.18 mm respectively and Streptococcus epidermidis (19.07; 19.21; 20.22; 21.62; 40.00 mm respectively). Ethanolic 96% extract and ethyl acetate fraction of ethanolic extract of Nigella sativa have antibacterial effect toward Staphylococcus aureus and Streptococcus epidermidis invitro.
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