Antibacterial effect of eucalyptus (Melaleuca leucadendra) ecoenzyme produced by Lamongan’s MSMEs against Salmonella Typhi

Abstract

Typhoid fever is a disease with extensive epidemiological distribution, causing high morbidity and mortality. The lack of effectiveness in prevention and treatment contributes to its continued mass occurrence. Salmonella Typhi bacteria are the main etiological cause of typhoid fever and have demonstrated resistance to various typhoidal antibiotics. This study aims to explore alternative possibilities for these issues, both curatively and preventively. Eucalyptus (Melaleuca leucadendra) is known to contain numerous antibacterial compounds, with 1.8 cineole as the main antibacterial compound. This compound exerts antibacterial effects by disrupting bacterial cell membranes and walls. Ecoenzyme are also recognized for their antibacterial effects, containing antibacterial compounds such as acetic acid, citric acid, lipase enzyme, trypsin enzyme, and amylase enzymes. This research is a laboratory experimental study conducted using the agar-well diffusion method on Mueller Hinton agar media, with the diameter of the inhibition zone as the test result. The findings indicate that all tested concentrations of eucalyptus ecoenzyme (10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%) show no inhibitory zone in all three test replications. This result suggests that eucalyptus (Melaleuca leucadendra) ecoenzyme produced by Lamongan’s MSMEs does not exhibit antibacterial effects on Salmonella Typhi.