Antibacterial Effect of Dental Adhesive Containing Dimethylaminododecyl Methacrylate on the Development of Streptococcus mutans Biofilm

Suping Wang,Keke Zhang,Xuedong Zhou,Yang Ge,Xin Xu,Hockin Xu,Shida Wang,Michael Weir,Mingyun Li,Yuqing Li,Lei Cheng,Ning Xu

doi:10.3390/ijms150712791

Suping Wang, Keke Zhang + Show 10 more

Open Access

https://doi.org/10.3390/ijms150712791

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Abstract

Antibacterial bonding agents and composites containing dimethylaminododecyl methacrylate (DMADDM) have been recently developed. The objectives of this study were to investigate the antibacterial effect of novel adhesives containing different mass fractions of DMADDM on Streptococcus mutans (S. mutans) biofilm at different developmental stages. Different mass fractions of DMADDM were incorporated into adhesives and S. mutans biofilm at different developmetal stages were analyzed by MTT assays, lactic acid measurement, confocal laser scanning microscopy and scanning electron microscopy observations. Exopolysaccharides (EPS) staining was used to analyze the inhibitory effect of DMADDM on the biofilm extracellular matrix. Dentin microtensile strengths were also measured. Cured adhesives containing DMADDM could greatly reduce metabolic activity and lactic acid production during the development of S. mutans biofilms (p < 0.05). In earlier stages of biofilm development, there were no significant differences of inhibitory effects between the 2.5% DMADDM and 5% DMADDM group. However, after 72 h, the anti-biofilm effects of adhesives containing 5% DMADDM were significantly stronger than any other group. Incorporation of DMADDM into adhesive did not adversely affect dentin bond strength. In conclusion, adhesives containing DMADDM inhibited the growth, lactic acid production and EPS metabolism of S. mutans biofilm at different stages, with no adverse effect on its dentin adhesive bond strength. The bonding agents have the potential to control dental biofilms and combat tooth decay, and DMADDM is promising for use in a wide range of dental adhesive systems and restoratives.

Highlights

IntroductionDental caries are considered one of the most prevalent chronic diseases of people worldwide [1]
Dental caries are considered one of the most prevalent chronic diseases of people worldwide [1].Caries are still a heavy burden around the world though the disease process and the potential to prevent and control the disease are much better understood [1,2]
In the tested mass fraction, the results demonstrate that incorporation of dimethylaminododecyl methacrylate (DMADDM) into adhesives did not adversely affect the dentin microtensile strengths

Summary

Introduction

Dental caries are considered one of the most prevalent chronic diseases of people worldwide [1]. Caries are still a heavy burden around the world though the disease process and the potential to prevent and control the disease are much better understood [1,2]. Resin composites are becoming popular filling materials due to their esthetics and direct-filling capabilities in the treatment of dental caries [3,4,5,6,7,8,9,10]. Half of all restorations fail within 10 years, and replacing them consumes 50% to 70% of the dentists’ time [11]. Previous investigation indicated that secondary caries is still a great challenge for the dental restoration [7]

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Discussion

Conclusion