Antibacterial Chitosan Nanofiber Thin Films with Bacitracin Zinc Salt.

Kazutaka Kumamoto,Yuki Shirosaki,Satoshi Hayakawa,Meng-Jiy Wang,Toshinari Maeda,Nurul Asyifah Binti Mustapha

doi:10.3390/polym13071104

Abstract

Chitosan nanofiber has a highly uniform structure of 20–50 nm in diameter and shows high dispersibility in water due to its submicron size and high surface-to-volume ratio. The stacked nanofibers film is useful for breathability because it has a gap with a size of several tens of nm or more. However, the chemical bonds between the nanofibers cannot be broken during use. In this study, the thin films were obtained by filtration of chitosan nanofibers and 3-glycidoxypropyltrimethoxysilane (GPTMS) mixture. The addition of GPTMS changed the wettability, mechanical property and stability in water of the thin films. Bacitracin zinc salt (BZ) has been used for the localized dermatological medicines and loaded in the films. BZ interacted electrostatically with the thin films matrix and the release of BZ was controlled by the amount of GPTMS. A higher released amount of BZ showed higher antibacterial effects toward S. aureus. The film was also tested their toxicity by L929 fibroblasts. The release of less than 11.9 μg of BZ showed antibacterial effects, but were not toxic for fibroblast cells.

Highlights

Staphylococcus aureus (S. aureus, a Gram-positive bacteria) is a major bacterial human pathogen related to skin infections [1,2,3]
We focused on the combination of chitosan nanofibers and silane coupling agent
The results suggest that the percentage of free amino groups was 30% in ChG01, 20% in ChG05 and 10% in ChG10

Summary

Introduction

Staphylococcus aureus (S. aureus, a Gram-positive bacteria) is a major bacterial human pathogen related to skin infections [1,2,3]. Most skin infections are not life-threatening but can cause psychological stress. Developed antibiotics do not generally cause these serious side effects, but they should generally not be taken with other medicines. Even minor side effects can cause distress over these long periods. In the case of severe vulgaris, the condition may not be completely cured and may remain as a mark accompanied by ridges, depressions and pigmentation. Patients may leave treatments unfinished because of the long-term nature of the battle with bacteria. For these reasons, it is critical to prevent S. aureus-derived skin infections in a facile manner using simple materials, to maintain a high quality of life for patients

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