Abstract
Juglans sigillata Dode., a fast growing deciduous tree species in the family Juglandaceae, is indigenous to mountain slopes and valleys of Tibet, Yunnan, Sichuan, and Guizhou provinces of southwest China [1, 2]. The green husk, cortex, kernel, nutshell, root and leaf of J. sigillata have a long history of being used in folk medicines to treat oxidative, inflammatory, rheumatic and nociceptive diseases, as well as to relief eczema, cancer, kidneys and stomach disorders. In the present study, antibactierial properties of J. sigillata green husk extractives were studied by hole-plate diffusion assay method described by Rios et al. [3] against Gram-negative bacteria, including Salmonella enterica and Escherichia coli and Gram-positive bacteria, such as Bacillus subtilis and Staphylococcus aureus. Aqueous EtOH (95%, v/v) extracts from green husk were successively partitioned with a serious of polar solvents to get fractions soluble in n-hexane, CH2Cl2, EtOAc, n-BuOH and H2O. Results, expressed by diameter of inhibition zone, revealed that the 95% aqueous EtOH extracts and all resulting soluble fractions from J. sigillata green husk revealed moderate or significant antibacterial effects against the four Gram bacteria, which indicated that J. sigillata green husk extractives have potential to destroy bacteria or suppress their growth or their ability to reproduce and could be used as excellent antibacterial agents.
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