Antibacterial behavior of organosilicon composite with nano aluminum oxide without influencing animal cells

Abstract

A technology for producing a nanocomposite based on the borsiloxane polymer and aluminum oxide nanoparticles (Al2O3 NPs) has been developed. Nanocomposites containing 0.001, 0.01, and 0.1 wt% Al2O3 NPs have been created. It has been shown that the nanocomposite with any content of Al2O3 NPs did not lose the main rheological properties of borsiloxane. The resulting nanomaterial is capable of generating reactive oxygen species (ROS) such as hydrogen peroxide and hydroxyl radicals. The rate of ROS generation increases up to 3 times with an increase in the concentration of Al2O3 NPs. It has been shown that the nanocomposite leads to the formation of long-lived reactive protein species, and is also the reason for the appearance of such a key biomarker of oxidative stress as 8-oxoguanine in DNA. The intensity of the process increases with an increase in the concentration of Al2O3 NPs. Moreover, it was found that bacterial cells adhere to the surfaces of the nanocomposite (reduces the number of bacteria on the substrate by 39 times), and the nanocomposite can detach bacterial cells not only from the surfaces, but also from wetted substrates. The ability to capture bacterial cells is primarily associated with the properties of the polymer; they are weakly affected Al2O3 NPs. The nanocomposite is non-toxic to eukaryotic cells, the surface of the nanocomposite is suitable for eukaryotic cells for colonization. Due to unique physical properties, low cytotoxicity and antimicrobial action, this nanocomposite can be used in medicine and prosthetics.