Abstract
Many methods have been used for the preparation of nanostructured metal oxides. Here we report the synthesis of TiO2 nanoparticles by facile hydrothermal process by varying the concentration of the precursor and reaction temperature while keeping the process time constant. Morphological, structural and optical studies were carried out by scanning electron microscopy equipped with energy dispersive spectroscopy, X-ray powder diffraction spectroscopy and VU-Vis-NIR spectroscopy. Morphological and compositional analysis reveal that the prepared nanoparticles are highly pure with an approximate average particle size 5-15nm. XRD studies showed their crystalline structure and the sizes around 5 nm, while the optical absorption studies in the photon wavelength range 300-600 nm reveal that the strong absorbance peak is positioned at around 3.5 eV nm whereas visible energy is almost transparent for the materials. Finally, the antibacterial effect of TiO2 nanoparticles has been studied. Plating technique was used to determine lowest concentration that prevent or inhibit growth of bacteria. This technique of TiO2 nanoparticle was used against most common organisms which cause wound infection including: MRSA, E. coli and Pseudomonas aeruginosa. Different concentrations of TiO2 nanoparticles were used 100 μg/ml, 200 μg/ml, 400 μg/ml, 600 μg/ml and 800 μg/ml. Inhibition of bacteria was different for prepared TiO2 samples due to different concentration of the precursors and synthesis temperature.
Highlights
In the recent past, nanomaterials of different metal oxides have attracted much attention due to their variety of technological application they offer such as, catalysis, sensors, optoelectronics applications, solar cells, electroluminescent devices, light-emitting devices and environmental remediation [1,2,3,4,5,6,7]
The antimicrobial activity of bacterial strains against a range of known concentration of TiO2 nanocrystallites after a period of time intervals was evaluated by counting the CFUs/ml to detect to minimum inhibitory concentration (MIC)
Dose Response Curve of bacteria that was treated with TiO2 nanoparticles indicated that, inhibitory effect of TiO2 nanoparticles was different between species and from sample to sample
Summary
Nanomaterials of different metal oxides have attracted much attention due to their variety of technological application they offer such as, catalysis, sensors, optoelectronics applications, solar cells, electroluminescent devices, light-emitting devices and environmental remediation [1,2,3,4,5,6,7]. The NPs are coated in the form of oxides to prevent bacterial proliferation and colonization due to their catalytic activity They have successfully been exploited for the detection of proteins, production of drugs, purification and separation of cells and biological molecules and for the cure of tumors [11]. TiO2 NPs have been successfully synthesized by several methods, for example, sol-gel, precipitation, electrochemical, solvothermal, sonochemical, solid state reactions and microwave irradiation technique [15,16,17,18,19,20,21] These processes involve environmentally unfriendly reagents which may cause biological hazards or toxicity. The impact of this work will give new strategies in treatment of wound infection
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
